Steven Finn has been ruled of the second Test against India in Mumbai after aggravating the thigh injury he suffered early in the tour.
Finn was injured during England's first warm-up match of the trip and bowled just four overs. He will now undergo a further scan in Mumbai on Wednesday to determine the extent of the problem.
Andy Flower, the England team director, said: "Steven has been working hard to get back to full fitness. He has unfortunately hurt his leg again, a couple of days ago, and is having another scan tomorrow.
"We don't think he's got any structural damage, but it would be careless to suggest that he might play the second Test."
However, Flower remained hopeful Finn could still play a part in the series and suggested they may use the Performance Squad, which arrived in Mumbai on Tuesday, to help in his recovery.
"They've got a three-day game starting on the 27th," Flower said. "If he's ready for that, which is the last day of the second Test, we'll put him through his paces. If he comes through that well, he'll be available for the third and fourth Tests.
"That extra pace would certainly assist us. But that's not to be, so let's hope he gets fit and ready for that third Test. I've got an open mind about it. Let's see what his scan reveals tomorrow. We hope he goes and plays that three-day game, and comes through well."
Finn had been tipped to be recalled for the second Test to bring a cutting edge to England's pace attack which struggled to make an impression in the opening Test in Ahmedabad. James Anderson, Stuart Broad and Tim Bresnan managed just one wicket between them and were considerably out-bowled by India's Zaheer Khan and Umesh Yadav.
There is still a strong chance that England will make changes to their bowling attack with Monty Panesar tipped for a call-up.
"We'll assess conditions tomorrow, when we're down at the ground for training," Flower said. "It would be naive, or silly, to prejudge them without actually having seen the pitch.
"But it might be fairly obvious that it might turn a little - which is good, because I'd like our guys to test themselves against the turning ball again here in Mumbai. Monty Panesar, of course, has a chance to play.
"We might well go with two spinners, but we'll judge that when we actually see the conditions. Of course these are challenging times for selectors, coaches, players. That's why we're here, because we like putting ourselves in those positions. If we didn't like to make those sorts of decisions; if we didn't like being in an arena where you're tested, and you're judged, then we wouldn't be here."
There will be an enforced change in the batting after Ian Bell flew home for the birth of his first son, Joseph William, but he did not make it home in time for the delivery.