Having wrapped up the one-day series against West Indies with victory at The Oval, England have taken the opportunity to rest Stuart Broad, Tim Bresnan and Graeme Swann from the final match at Headingley on Friday.
Stuart Meaker, the Surrey fast bowler, Warwickshire allrounder Chris Woakes and Kent offspinner James Tredwell have been added into the squad. Woakes had already been drafted in as cover after Jade Dernbach was given compassionate leave following the death of Tom Maynard on Monday but Dernbach remains in the squad for the final ODI.
Broad, who is the Twenty20 captain, was also rested for the final Test against West Indies at Edgbaston but for Bresnan and Swann, both part of the Twenty20 squad, it is the first England match of the season they will miss. Since the retirement from one-day cricket of Kevin Pietersen this trio are the only first-choice selections in all three formats.
The players called into the squad all have previous ODI experience with Meaker making his debut against India last October and playing two matches in the series. Woakes, who was originally on that tour before pulling out injured, has played four matches which include taking 6 for 45 against Australia at Brisbane, the second-best figures for England in ODIs.
Tredwell, meanwhile, was the reserve spinner in the Test squad for the tour of Sri Lanka earlier this year and last appeared in a one-day international in the World Cup quarter-final against Sri Lanka in Colombo. In the game before that, against West Indies, he took a match-winning 4 for 48 to keep England alive in the tournament.
Geoff Miller, the national selector, said: "The fact that we have already won the NatWest series means we are able to take the opportunity to rest three players ahead of next week's series against Australia and take a closer look at players who are likely to feature in our limited overs planning going forward."
England played the same side in both the first two matches, winning by 114 runs at West End and eight wickets at The Oval, meaning Dernbach, Samit Patel and Jonny Bairstow from the original squad have yet to feature.
Revised squad Alastair Cook (capt), Ian Bell, Jonathan Trott, Ravi Bopara, Eoin Morgan, Craig Kieswetter, Jonny Bairstow, Samit Patel, Chris Woakes, James Tredwell, James Anderson, Steven Finn, Jade Dernbach, Stuart Meaker